{"title":"TIRF","description":"\u003cdiv class=\"flex flex-col text-sm pb-25\"\u003e\n\u003carticle class=\"text-token-text-primary w-full focus:outline-none [--shadow-height:45px] has-data-writing-block:pointer-events-none has-data-writing-block:-mt-(--shadow-height) has-data-writing-block:pt-(--shadow-height) [\u0026amp;:has([data-writing-block])\u0026gt;*]:pointer-events-auto scroll-mt-[calc(var(--header-height)+min(200px,max(70px,20svh)))]\" dir=\"auto\" data-turn-id=\"request-693aee33-2c98-832b-ba4c-79c5b6dd2d60-3\" data-testid=\"conversation-turn-26\" data-scroll-anchor=\"true\" data-turn=\"assistant\" tabindex=\"-1\"\u003e\n\u003cdiv class=\"text-base my-auto mx-auto pb-10 [--thread-content-margin:--spacing(4)] @w-sm\/main:[--thread-content-margin:--spacing(6)] @w-lg\/main:[--thread-content-margin:--spacing(16)] px-(--thread-content-margin)\"\u003e\n\u003cdiv class=\"[--thread-content-max-width:40rem] @w-lg\/main:[--thread-content-max-width:48rem] mx-auto max-w-(--thread-content-max-width) flex-1 group\/turn-messages focus-visible:outline-hidden relative flex w-full min-w-0 flex-col agent-turn\" tabindex=\"-1\"\u003e\n\u003cdiv class=\"flex max-w-full flex-col grow\"\u003e\n\u003cdiv data-message-author-role=\"assistant\" data-message-id=\"6d1d19b0-6f1a-447c-a58b-29a85d0bfff9\" dir=\"auto\" class=\"min-h-8 text-message relative flex w-full flex-col items-end gap-2 text-start break-words whitespace-normal [.text-message+\u0026amp;]:mt-1\" data-message-model-slug=\"gpt-5-2\"\u003e\n\u003cdiv class=\"flex w-full flex-col gap-1 empty:hidden first:pt-[1px]\"\u003e\n\u003cdiv class=\"markdown prose dark:prose-invert w-full break-words dark markdown-new-styling\"\u003e\n\u003cp data-start=\"0\" data-end=\"203\" data-is-last-node=\"\" data-is-only-node=\"\"\u003eTotal Internal Reflection Fluorescence microscopy enables selective excitation of fluorophores at the cell surface. You gain high contrast imaging of membrane level events with minimal background signal.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"z-0 flex min-h-[46px] justify-start\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"mt-3 w-full empty:hidden\"\u003e\n\u003cdiv class=\"text-center\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/article\u003e\n\u003c\/div\u003e","products":[{"product_id":"89-north-optotirf-motorized-tirf-image-splitter-89n-s1380-tir-unt","title":"89 North OptoTIRF Motorized TIRF Image Splitter","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe 89 North OptoTIRF Motorized TIRF system (Model: 89N-S1380\/TIR\/UNT) is a cutting-edge illumination module designed for high-precision Total Internal Reflection Fluorescence (TIRF) microscopy. This motorized platform allows for dynamic control of the incidence angle and laser steering, offering exceptional spatial and temporal resolution for live-cell and single-molecule imaging. It integrates seamlessly with modern microscope systems and is ideal for labs performing advanced fluorescence and surface-level studies.\u003c\/p\u003e\n\n\u003ch2\u003eCondition\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eCondition:\u003c\/strong\u003e New with Manufacturer Warranty\u003c\/p\u003e\n\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMotorized TIRF launch for precise control of incidence angle\u003c\/li\u003e\n  \u003cli\u003eCompatible with single and multi-wavelength laser systems\u003c\/li\u003e\n  \u003cli\u003eSupports live-cell, membrane, and single-molecule imaging\u003c\/li\u003e\n  \u003cli\u003eDesigned for easy alignment and stable long-term performance\u003c\/li\u003e\n  \u003cli\u003eRemote and software control for reproducible experimental setups\u003c\/li\u003e\n  \u003cli\u003eCompact form factor for easy integration into existing microscope systems\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eTotal Internal Reflection Fluorescence (TIRF) microscopy\u003c\/li\u003e\n  \u003cli\u003eSingle-molecule localization microscopy (SMLM)\u003c\/li\u003e\n  \u003cli\u003eLive-cell membrane dynamics\u003c\/li\u003e\n  \u003cli\u003eSuper-resolution imaging platforms\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" cellpadding=\"6\" cellspacing=\"0\"\u003e\n  \u003cthead\u003e\n    \u003ctr\u003e\n      \u003cth\u003eSpecification\u003c\/th\u003e\n      \u003cth\u003eDetails\u003c\/th\u003e\n    \u003c\/tr\u003e\n  \u003c\/thead\u003e\n  \u003ctbody\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eManufacturer\u003c\/td\u003e\n      \u003ctd\u003e89 North\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eModel\u003c\/td\u003e\n      \u003ctd\u003eOptoTIRF (89N-S1380\/TIR\/UNT)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eSystem Type\u003c\/td\u003e\n      \u003ctd\u003eMotorized TIRF Launch System\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eControl\u003c\/td\u003e\n      \u003ctd\u003eSoftware and remote-controlled motorized adjustments\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eApplication\u003c\/td\u003e\n      \u003ctd\u003eTIRF, single-molecule, and super-resolution fluorescence imaging\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eCompatibility\u003c\/td\u003e\n      \u003ctd\u003eIntegrates with major microscope brands and laser sources\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eStatus\u003c\/td\u003e\n      \u003ctd\u003eNew with Manufacturer Warranty\u003c\/td\u003e\n    \u003c\/tr\u003e\n  \u003c\/tbody\u003e\n\u003c\/table\u003e\n","brand":"89 North","offers":[{"title":"Default Title","offer_id":45376862257406,"sku":"89N-S1380\/TIR\/UNT","price":14750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0693\/7860\/5310\/files\/89N-S1380_TIR_UNT_1-Photoroom_ff405ab9-d51c-466b-b55d-e87e7baa212c.png?v=1766519226"},{"product_id":"zeiss-tirf-system-w-axio-observer-z1-w-optional-definite-focus-1-2-or-3-tirf-51653","title":"Zeiss TIRF System w\/ Axio Observer Z1 w\/ Optional Definite Focus (1, 2, or 3) TIRF","description":"\u003ch2\u003eZeiss TIRF System with Axio Observer Z1 Inverted Microscope | Motorized, Phase Contrast, Fluorescence, Optional Definite Focus (1, 2, or 3)\u003c\/h2\u003e\n\n\u003cp\u003eThis advanced Zeiss Axio Observer Z1 system is configured with a full Total Internal Reflection Fluorescence (TIRF) imaging package, ideal for single-molecule studies, membrane dynamics, and near-membrane cellular processes. The system features motorization, phase contrast, optional Definite Focus (1, 2, or 3), and includes all key components required for high-performance TIRF microscopy. This is a turnkey platform for TIRF imaging workflows using laser excitation and precision angle adjustment.\u003c\/p\u003e\n\n\u003ch3\u003eCondition\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eRefurbished\/Used\u003c\/strong\u003e\u003cbr\u003e\n(This product has been inspected and verified to meet manufacturer specifications.)\u003c\/p\u003e\n\n\u003ch3\u003eIncluded TIRF System Components\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eZeiss TIRF Module (P\/N 428300-8201)\u003c\/li\u003e\n  \u003cli\u003eZeiss Lasermodul TIRF (P\/N 1514-469)\u003c\/li\u003e\n  \u003cli\u003eZeiss Laser Control System\u003c\/li\u003e\n  \u003cli\u003eZeiss Laser Manipulation Controller (P\/N 42635-9000)\u003c\/li\u003e\n  \u003cli\u003eZeiss Zen Blue Software Dongle\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThese components enable precision control over laser incidence angle and full integration with Zeiss Zen software for automated imaging, making this system ready for advanced TIRF applications out of the box.\u003c\/p\u003e\n\n\u003ch3\u003eKey Features\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eMicroscope Frame: Zeiss Axio Observer Z1 (Inverted)\u003c\/li\u003e\n  \u003cli\u003eIntegrated TIRF Imaging System with Laser Control and Manipulation\u003c\/li\u003e\n  \u003cli\u003ePhase Contrast Capability\u003c\/li\u003e\n  \u003cli\u003eMotorized Stand and Optical Components\u003c\/li\u003e\n  \u003cli\u003eOptional Support for Zeiss Definite Focus 1, 2, or 3\u003c\/li\u003e\n  \u003cli\u003eZen Blue Software Integration (Dongle Included)\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eTotal Internal Reflection Fluorescence (TIRF) Microscopy\u003c\/li\u003e\n  \u003cli\u003eSingle Molecule Fluorescence\u003c\/li\u003e\n  \u003cli\u003eCell Membrane and Adhesion Studies\u003c\/li\u003e\n  \u003cli\u003eLive-Cell Near-Surface Imaging\u003c\/li\u003e\n  \u003cli\u003eSuper-Resolution Microscopy Foundations\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003ctable\u003e\n  \u003cthead\u003e\n    \u003ctr\u003e\n      \u003cth\u003eComponent\u003c\/th\u003e\n      \u003cth\u003eSpecification \/ Details\u003c\/th\u003e\n    \u003c\/tr\u003e\n  \u003c\/thead\u003e\n  \u003ctbody\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eMicroscope Frame\u003c\/td\u003e\n      \u003ctd\u003eZeiss Axio Observer Z1, Inverted\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eImaging Modes\u003c\/td\u003e\n      \u003ctd\u003eTIRF, Fluorescence, Phase Contrast\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eTIRF Hardware\u003c\/td\u003e\n      \u003ctd\u003eIncluded (Module, Lasermodul, Laser Control, Manipulation Module)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eLaser Control\u003c\/td\u003e\n      \u003ctd\u003eZeiss Laser Control + Laser Manipulation Module\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eSoftware\u003c\/td\u003e\n      \u003ctd\u003eZeiss Zen Blue with Dongle\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eAutofocus Compatibility\u003c\/td\u003e\n      \u003ctd\u003eDefinite Focus 1, 2, or 3 (optional)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eMotorization\u003c\/td\u003e\n      \u003ctd\u003eMotorized Stand and Optical Components (varies by configuration)\u003c\/td\u003e\n    \u003c\/tr\u003e\n  \u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003ch3\u003eMicroscope Configuration Options\u003c\/h3\u003e\n\n\u003ch4\u003eObjectives\u003c\/h4\u003e\n\u003cp\u003ePlease discuss your application or preferred configuration with our team. We have a full suite of options available.\u003c\/p\u003e\n\n\u003ch4\u003eStage\u003c\/h4\u003e\n\u003cp\u003eMotorized and Manual options available.\u003c\/p\u003e\n\n\u003ch4\u003eNosepiece\u003c\/h4\u003e\n\u003cp\u003eSpecify type and size. Typically configured with a 6-position motorized or manual nosepiece.\u003c\/p\u003e\n\n\u003ch4\u003eFilter Cubes\u003c\/h4\u003e\n\u003cp\u003eIncludes (3) filter cubes of your choice.\u003cbr\u003e\nAdditional filter cubes available for purchase.\u003c\/p\u003e\n\n\u003ch4\u003eIlluminator (Choose One):\u003c\/h4\u003e\n\u003cul\u003e\n  \u003cli\u003e100w Mercury w\/ Power Supply\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eUpgrade to:\u003c\/strong\u003e Used Metal Halide\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eUpgrade to:\u003c\/strong\u003e New X-Cite 110LED System\n    \u003cul\u003e\n      \u003cli\u003eIncludes: LED Head, miniCUBE Controller, speedDIAL Remote\u003c\/li\u003e\n      \u003cli\u003e3mm x 1500mm Liquid Light Guide\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eUpgrade to:\u003c\/strong\u003e New X-Cite mini+ System\n    \u003cul\u003e\n      \u003cli\u003eIncludes: LED Head, miniCUBE Controller, speedDIAL Remote\u003c\/li\u003e\n      \u003cli\u003eMicroscope Coupling Flange\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eUpgrade to:\u003c\/strong\u003e New X-Cite XYLIS II, XT730S\n    \u003cul\u003e\n      \u003cli\u003eIncludes: LED Light Engine, speedDIAL Remote\u003c\/li\u003e\n      \u003cli\u003e3mm x 1500mm Liquid Light Guide\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cstrong\u003eConfiguration shown is for demonstration purposes. Contact us to discuss your application and needs for a customized quote.\u003c\/strong\u003e\u003c\/p\u003e\n","brand":"ReUse - University of Minnesota","offers":[{"title":"Default Title","offer_id":47401212412158,"sku":"51653","price":66000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0693\/7860\/5310\/files\/Zeiss_Z1_51653_System_A_dd8a2c84-a9f7-46f8-ac4a-fccb6d21a887.png?v=1768836543"},{"product_id":"visitron-orbital-100-ring-tirf-tirf-vv-orbital-100ringtirf","title":"Visitron ORBITAL 100 Ring-TIRF TIRF","description":"\u003ch2\u003eVisitron Systems ORBITAL 100 Ring-TIRF – ORBITAL-RINGTIRF\u003c\/h2\u003e\n\n\u003cp\u003eAchieve unparalleled control of evanescent illumination with the Visitron Systems ORBITAL 100 Ring-TIRF system. Designed for advanced live-cell imaging applications, the ORBITAL 100 employs a unique beam-orbiting mechanism that delivers ultra-uniform TIRF illumination across the entire field of view. Its fast, software-controlled rotation eliminates photobleaching hotspots and ensures reproducibility in dynamic fluorescence imaging.\u003c\/p\u003e\n\n\u003ch3\u003eCondition\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eNew with Manufacturer Warranty\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003ch3\u003eKey Features\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBeam-Orbiting TIRF System:\u003c\/strong\u003e Creates circular beam movement to achieve exceptional field uniformity without mechanical stage movement.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eMulti-Channel Imaging Support:\u003c\/strong\u003e Easily couples to standard laser combiners and fiber systems to enable multi-wavelength excitation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDynamic Control:\u003c\/strong\u003e Motorized ring size and rotation speed are software-controlled, allowing real-time optimization during experiments.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDual Illumination Modes:\u003c\/strong\u003e Switch seamlessly between Total Internal Reflection (TIRF) and Highly Inclined Laminated Optical sheet (HILO) illumination.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eVisiView Software Integration:\u003c\/strong\u003e Native control via Visitron’s VisiView or third-party platforms including μManager and MetaMorph.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCompact Integration:\u003c\/strong\u003e Mounts directly to the microscope’s rear or side port without interfering with other hardware.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eSuper-resolution microscopy (e.g., STORM, PALM)\u003c\/li\u003e\n  \u003cli\u003eSingle-molecule tracking\u003c\/li\u003e\n  \u003cli\u003eLive-cell membrane dynamics\u003c\/li\u003e\n  \u003cli\u003eEndocytosis and exocytosis studies\u003c\/li\u003e\n  \u003cli\u003eReceptor-ligand interactions\u003c\/li\u003e\n  \u003cli\u003eHigh-speed fluorescence imaging\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003ctable\u003e\n  \u003cthead\u003e\n    \u003ctr\u003e\n      \u003cth\u003eSpecification\u003c\/th\u003e\n      \u003cth\u003eDetails\u003c\/th\u003e\n    \u003c\/tr\u003e\n  \u003c\/thead\u003e\n  \u003ctbody\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eIllumination Method\u003c\/td\u003e\n      \u003ctd\u003eRing-scanned TIRF (beam orbiting with precision control)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eMaximum Beam Rotation Speed\u003c\/td\u003e\n      \u003ctd\u003eUp to 100 revolutions per second\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eBeam Positioning\u003c\/td\u003e\n      \u003ctd\u003eMotorized XY control\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eRing Diameter Adjustment\u003c\/td\u003e\n      \u003ctd\u003eMotorized, continuous\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eTIRF Modes\u003c\/td\u003e\n      \u003ctd\u003eTIRF and Near-TIRF (HILO)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eIllumination Wavelength Range\u003c\/td\u003e\n      \u003ctd\u003e400–700 nm (dependent on laser system)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eExcitation Source Compatibility\u003c\/td\u003e\n      \u003ctd\u003eSingle or multi-line fiber-coupled laser combiners\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eMounting Interface\u003c\/td\u003e\n      \u003ctd\u003eC-mount \/ Microscope-specific adapters available\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eSoftware Integration\u003c\/td\u003e\n      \u003ctd\u003eFull control via VisiView, MetaMorph, μManager\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eControl Interface\u003c\/td\u003e\n      \u003ctd\u003eUSB-controlled external motor driver\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eField Uniformity\u003c\/td\u003e\n      \u003ctd\u003eOptimized for 60x\/100x high-NA objectives\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eSystem Alignment\u003c\/td\u003e\n      \u003ctd\u003eIntegrated alignment aid with beam visualization mode\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eDimensions (Controller)\u003c\/td\u003e\n      \u003ctd\u003eApprox. 160 x 105 x 45 mm\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003ePower Requirements\u003c\/td\u003e\n      \u003ctd\u003e12V DC (power supply included)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eSupported Microscopes\u003c\/td\u003e\n      \u003ctd\u003eCompatible with major inverted fluorescence microscope platforms\u003c\/td\u003e\n    \u003c\/tr\u003e\n  \u003c\/tbody\u003e\n\u003c\/table\u003e\n","brand":"Visitron","offers":[{"title":"Default Title","offer_id":47637952561406,"sku":"VV-Orbital-100RingTIRF","price":0.0,"currency_code":"USD","in_stock":true}]},{"product_id":"visitron-orbital-110-isotirf-ring-tirf-combined-with-vs-homogenizer-technology-tirf-vv-orbital110isotirf","title":"Visitron ORBITAL-110 IsoTIRF Ring-TIRF combined with VS-Homogenizer Technology TIRF","description":"\u003ch2\u003eVisitron Systems ORBITAL-110 IsoTIRF – Ring-TIRF with VS-Homogenizer Technology\u003c\/h2\u003e\n\n\u003cp\u003eThe Visitron ORBITAL-110 IsoTIRF system combines advanced Ring-TIRF laser illumination with integrated VS-Homogenizer optics to deliver industry-leading excitation uniformity. This compact, high-performance system eliminates Gaussian beam artifacts by producing a flat-field evanescent illumination profile—ideal for high-sensitivity live-cell imaging, super-resolution, and single-molecule applications without the need for mathematical shading correction.\u003c\/p\u003e\n\n\u003ch3\u003eCondition\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eNew with Manufacturer Warranty\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003ch3\u003eKey Features\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eIsoTIRF Homogenizer Optics:\u003c\/strong\u003e Achieves a uniform intensity profile across the full field of view—less than 5% roll-off variation across wavelengths (405–640 nm).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eRing-TIRF Beam Orbiting:\u003c\/strong\u003e Galvo-driven 360° elliptical beam scanning minimizes interference fringes and improves illumination homogeneity.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eMulti-Wavelength Uniformity:\u003c\/strong\u003e Consistent performance across all major excitation lines (405 nm, 488 nm, 561 nm, and 640 nm).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eModular Design:\u003c\/strong\u003e Upgradeable from Orbital-100 platform; integrates LED or white light illumination for widefield\/epifluorescence imaging.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCompact Footprint:\u003c\/strong\u003e Integrates easily with inverted microscope systems and laser combiners using polarization-maintaining fiber input.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFully Software Controlled:\u003c\/strong\u003e Seamless integration with VisiView for mode switching, beam path control, angle tuning, and time-sharing support.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eSingle-molecule localization microscopy (PALM, STORM)\u003c\/li\u003e\n  \u003cli\u003eLive-cell TIRF imaging\u003c\/li\u003e\n  \u003cli\u003eProtein and receptor tracking\u003c\/li\u003e\n  \u003cli\u003eKinetic studies of single-molecule interactions\u003c\/li\u003e\n  \u003cli\u003eSuper-resolution widefield techniques\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003ctable\u003e\n  \u003cthead\u003e\n    \u003ctr\u003e\n      \u003cth\u003eSpecification\u003c\/th\u003e\n      \u003cth\u003eDetails\u003c\/th\u003e\n    \u003c\/tr\u003e\n  \u003c\/thead\u003e\n  \u003ctbody\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eIllumination Method\u003c\/td\u003e\n      \u003ctd\u003eRing-TIRF with IsoTIRF Homogenizer Optics\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eBeam Movement\u003c\/td\u003e\n      \u003ctd\u003e2D Galvo-driven, circular or elliptical path at the objective's back focal plane\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eHomogeneity (Roll-Off)\u003c\/td\u003e\n      \u003ctd\u003e\u0026lt;5% with homogenizer optics (compared to 40–80% without)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eSupported Wavelengths\u003c\/td\u003e\n      \u003ctd\u003e405 nm, 488 nm, 561 nm, 640 nm\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eLaser Source Compatibility\u003c\/td\u003e\n      \u003ctd\u003eSingle-mode laser diodes (400–700 nm), fiber-coupled (polarization-maintaining)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eAvailable Illumination Modes\u003c\/td\u003e\n      \u003ctd\u003eRingTIRF, Single-Point TIRF, HILO, Oblique Illumination, Center Beam\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eControl Software\u003c\/td\u003e\n      \u003ctd\u003eVisiView (native integration), MetaMorph, μManager\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eSynchronization\u003c\/td\u003e\n      \u003ctd\u003eLaser shuttering and galvo scanning synced with camera exposure\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003ePenetration Depth Control\u003c\/td\u003e\n      \u003ctd\u003eCalibrated, slider-controlled TIRF angle adjustment\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eMounting Interface\u003c\/td\u003e\n      \u003ctd\u003eStandard microscope port or customized adapters\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003ePower Requirements\u003c\/td\u003e\n      \u003ctd\u003e12V DC (external power supply included)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eField of View Compatibility\u003c\/td\u003e\n      \u003ctd\u003eUp to 18.8 mm diagonal (tested with sCMOS 13.3 x 13.3 mm sensor)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd\u003eUpgradeable Platform\u003c\/td\u003e\n      \u003ctd\u003eScalable to Orbital-200, 500, and 600 systems\u003c\/td\u003e\n    \u003c\/tr\u003e\n  \u003c\/tbody\u003e\n\u003c\/table\u003e\n","brand":"Visitron","offers":[{"title":"Default Title","offer_id":47637952790782,"sku":"VV-ORBITAL110IsoTIRF","price":0.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/coastalmicroscopes.com\/collections\/tirf.oembed","provider":"Coastal Microscopes","version":"1.0","type":"link"}