BD Biosciences Accuri C6 Plus w/CSampler Plus 660517 Flow Cytometer

Includes Computer loaded with Software and C Sampler

The BD Accuri C6 Plus Flow Cytometer, with its compact 11 x 14.75 x 16.5-inch footprint, light weight of 30 lb and operational simplicity, supports a wide variety of applications including immunology, cell and cancer biology, plant and microbiology and industrial applications. This portable and compact cell analyzer holds the analytical power of a cytometer twice its size. Used in thousands of research studies, the BD Accuri C6 Plus System provides an easy-to-use option to your research toolbox.

The BD Accuri C6 Plus Flow Cytometer Advantages

Sensitive, reliable and capable

• Reliable instrument performance with automated QC
• Volumetric counting and continuous sampling

Simple: easy to learn, use, maintain and teach

• Quick and easy instrument setup
• Simplified workflow with BD quality reagents and kits
• Walkaway convenience with automated sample loader

Compact and transportable

• Small footprint for easy transportation between labs

Intuitive and easy-to-learn BD Accuri C6 Plus Software

• Intuitive user interface
• Easy to learn and data can be collected and analyzed in less than an hour
• Clearly visible software options and instrument controls

Specifications:

Optics

• Laser Excitation:488 nm, 640 nm
• Laser Profile: 10 x 75 μm
• Light Scatter DetectionForward (0°, ±13°)
• Side (90°, ±13°)
• Emission DetectionFour colors, user-changeable optical filters
• Standard set installed:
• FL1 533/30 nm (eg, FITC/GFP)
• FL2 585/40 nm (eg, PE/PI)
• FL3 >670 nm (eg, PerCP, PerCP-Cy5.5, PE-Cy™7)
• FL4 675/25 nm (eg, APC)
• Optical Alignment: Fixed alignment

Fluidics

• Flow Cell: 200-μm ID quartz capillary
• Minimum Detectable Particle Size: 0.5 μm
• Minimum Sample Volume: 50 μL
• Pre-Set Flow Rates and Core SizesSlow: 14 μL/min, 10-μm core
• Medium: 35 μL/min, 16-μm core
• Fast: 66 μL/min, 22-μm core
• Custom Sample Flow Rates: 10–100 μL/min
• Custom Core Diameter: 5–40 μm
• Recommended Sheath Fluid: 0.2-μm filtered DI water with BD™ Sheath Additive
• Maximum Events Per Sample: 1 million events
• Fluid Bottle Capacity:2-L sheath fluid
• 2-L waste
• 250-mL cleaner
• 250-mL decontamination fluid

Performance

• Fluorescence Sensitivity, MESF*: FITC <75; PE <50
• Scatter Resolution: Resolves human peripheral blood lymphocytes, monocytes
• and granulocytes
• Fluorescence Linearity: 2 ±0.05% for chicken erythrocyte nuclei (CEN)
• Fluorescence Precision: <3% CV for CEN
• Data Acquisition Rate: Up to 10,000 events/second
• Signal Processing: 24-bit data path

Data Management**

• Workstation Specifications: Dell OptiPlex 7450 All-in-One Desktop
• Processor and Memory
• Intel® i7 6700 3.4 GHz Quad Core, 8MB with HD graphics 530
• 8 GB (1 x 8 GB) 2,400 MHz DDR4
• Hard Drive and Data Storage Options
• 500-GB 2.5-inch Serial ATA (7,200 RPM)
• 8x Slimline DVD+/-RW Drive
• Display and USB Ports
• 23-inch Wide Viewing Angle, Full HD 1,920 x 1,080 resolution with anti-glare coating
• 8 USB ports (2 USB 3.0 side, 4 USB 3.0 and 2 USB 2.0 rear)
• Networking
• Intel Dual Band Wireless-8260 (802.11ac)
• Bluetooth 4.1
• Operating System: Microsoft® Windows® 7 Professional English, 64 bit
• BD Accuri C6 Plus Software Minimum Run Requirements
• 64-bit Microsoft® Windows® 7
• Minimum screen resolution 1,280 x 1,024
• 8 GB RAM
• CD or DVD ROM
• 26 GB of free hard disk space
• 3 USB 2.0 or USB 3.0 ports
• Computer Interface: USB 2.0 or USB 3.0

Installation Requirements

• Power Requirements: 100–240 VAC, 50/60 Hz
• Typical Power Consumption: 154 VA
• Heat Output: 240 BTU/hour maximum output
• Operating Ranges: 15–30°C; <80% relative humidity
• Instrument Size (H x W x D)
• 11 x 14.75 x 16.5 in. (27.9 x 37.5 x 41.9 cm)
• Footprint with Fluid Bottles (H x W x D)
• 11 x 21.5 x 16.5 in. (27.9 x 54.6 x 41.9 cm)
• Weight: 30 lb (13.6 kg)

BD CSampler Plus Accessory

• Power Requirements: No additional power necessary
• Software Requirements: BD CSampler Plus software
• Compatible Plate Types
• Standard 96-well plates (flat, round and v-bottom)
• Deep-well 96-well plates
• 48-well plates
• Plates manufactured to the guidelines published by the
• American National Standards Institute, submitted by the Society
• for Biomolecular Screening, should be compatible with the
• BD CSampler Plus.
• 12 x 75-mm tubes can also be accommodated using the
• supplied 24-tube rack.
• Space Requirements
• Minimum bench depth 28 in. (71 cm)
• Minimum width (cytometer with BD CSampler Plus) 19.5 in.
• (49.5 cm)
• Processing Time: <90 minutes for 96-well plate, utilizing 30-second acquisition time per well
• Weight: 7 lb (3.2 kg)
• Minimum Sample Volume: 50 μL for tubes or plates
• Wash Cycle: Up to 3 wash cycles per well
• Agitate Cycle: Up to 3 agitate cycles per well