{"product_id":"nikon-apo-tirf-dic-n2-100x-1-49-oil-m25-thread-size-microscope-objective-52351a","title":"Nikon Apo TIRF DIC N2 100x\/1.49 Oil M25 Thread Size Microscope Objective","description":"\u003ch2\u003eNikon CFI Apo TIRF 100x\/1.49 Oil DIC N2 Objective MRD01991 (Used)\u003c\/h2\u003e\n\u003cp\u003eThe Nikon CFI Apo TIRF 100x\/1.49 Oil is a high-NA apochromatic oil immersion objective for total internal reflection fluorescence (TIRF), single-molecule and super-resolution imaging. Its 1.49 NA allows objective-type TIRF illumination, and a correction collar with 23 °C and 37 °C scales matches cover glass thickness at room temperature or during live-cell work.\u003c\/p\u003e\n\u003ch2\u003eCondition\u003c\/h2\u003e\n\u003cp\u003eCondition: Refurbished\/Used (This product has been inspected and verified to meet manufacturer specifications)\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e100x magnification with 1.49 numerical aperture for objective-type TIRF\u003c\/li\u003e\n\u003cli\u003eApochromatic correction across the visible spectrum\u003c\/li\u003e\n\u003cli\u003eCorrection collar for 0.13 to 0.20 mm cover glass, with separate 23 °C and 37 °C scales\u003c\/li\u003e\n\u003cli\u003eDIC N2 rated for differential interference contrast\u003c\/li\u003e\n\u003cli\u003eOil immersion, 0.12 mm working distance\u003c\/li\u003e\n\u003cli\u003eCFI60 infinity optics, M25 thread, 60 mm parfocal distance\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTotal internal reflection fluorescence (TIRF) microscopy\u003c\/li\u003e\n\u003cli\u003eSingle-molecule imaging and tracking\u003c\/li\u003e\n\u003cli\u003eSuper-resolution localization microscopy (STORM, PALM)\u003c\/li\u003e\n\u003cli\u003eLive-cell imaging of membrane events at 37 °C\u003c\/li\u003e\n\u003cli\u003eHigh-resolution widefield fluorescence and DIC\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eManufacturer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNikon\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eModel\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCFI Apo TIRF 100x\/1.49 Oil\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCatalog number\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMRD01991\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMagnification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eNumerical aperture\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.49\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eImmersion\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOil\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWorking distance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.12 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCover glass\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.13 to 0.20 mm (correction collar, 23 °C and 37 °C scales)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCorrection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eApochromat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDIC\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eN2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOptical system\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCFI60, infinity corrected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eThread\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eM25\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParfocal distance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e60 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCompatible with\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNikon Eclipse Ti, Ti2 and other CFI60 microscopes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Nikon","offers":[{"title":"Default Title","offer_id":68046240776446,"sku":"52351a","price":8750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0693\/7860\/5310\/files\/Nikon_Apo_TIRF_100x_Objective_52351a_1.png?v=1794547114","url":"https:\/\/coastalmicroscopes.com\/products\/nikon-apo-tirf-dic-n2-100x-1-49-oil-m25-thread-size-microscope-objective-52351a","provider":"Coastal Microscopes","version":"1.0","type":"link"}