89 North North L-SPI Single Plane Illuminator Microscope Illuminator

Macro light sheet illuminator for 3D imaging of large samples

• Live-cell imaging with ultra-low phototoxicity
• Spatial and temporal fractionation of illumination
• Create up to 4 orthogonal lightsheets to reduce shadowing
• Long travel piezo stage for precise z-steps

The L-SPI is designed to work with any macro- or microscope, single-mode fiber laser source and scientific camera. This simple and effective instrument recombines two uniform, wide light sheets at right angles, reducing shadowing without the need for sample rotation or image fusion. The system includes optional sample chambers, fast long travel piezo stage and an optional second L-SPI head to bring sheets in from four directions. The L-SPI can be adapted to a wide range of experimental purposes. Its compact size and large freedom of access (e.g. for positioning micro-electrodes) make it a highly flexible solution.

Specifications:

• Number of sheets:2 or 4 orthogonal sheets possible
• Sheet thickness:
• 22 um with ~ 20 mm^2 imaging area
• 11 um with ~ 10 mm^2 imaging area
• Field of View:6 mm x 16 mm with 22 um thick sheet
• Z-Step size: 10 nm minimum step
• Z-travel range:20 mm
• Scan speed:Up to 10 mm/second