BD Biosciences FACSVia System w/ Loader 2 Lasers: Blue/Red

The BD FACSVia flow cytometer is small and lightweight, and can easily fit on any benchtop in the clinical laboratory, making the most of limited space. The system is equipped with a blue laser, a red laser, two light scatter detectors, and four fluorescence detectors. A compact optical design, fixed alignment, pre-optimized detector settings, and automated adjustment of fluorescence spillover (color compensation) work together to simplify workflow. A unique low-pressure pumping system drives the fluidics. A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration over 5 x 106 cells per µL. The BD FACSVia Loader accessory streamlines sample processing with reliable and easy-to-use automation.

The BD FACSVia Flow Cytometry System is an easy-to-use benchtop solution to help your laboratory run smoothly and efficiently.

• Automated instrument QC and compensation
• Intuitive user interface reduces manual steps
• Easy, low cost instrument maintenance minimizes operational costs to fit budget requirements
• Remote and onsite technical service ensures proper system support
• Bidirectional laboratory information system (LIS) communication minimizes potential transcription errors

BD FACSVia clinical software contains assay-specific templates providing clinical menus that include:

• BD Leucocount kit, designed for counting residual white blood cells (rWBCs) in leucoreduced blood products

Optics

• Laser excitation:488 nm, 640 nm
• Laser profile:
• 9 µm x 94 µm blue laser beam size
• 11 µm x 104 µm red laser beam size
• Light scatter detection:
• Forward (photodiode with 488/10 BP)
• Side (photodiode with 488/10 BP)
• Emission detection:
• Standard set optical filters installed:
• FL1 533/30 nm
• FL2 585/40 nm
• FL3 >670 nm**
• FL4 675/25 nm**
• Optical alignment: Fixed alignment

Performance

• Fluorescence sensitivity, MESF*: FITC <150; PE <100
• Scatter resolution: Resolves human peripheral blood lymphocytes, monocytes, and granulocytes
• Fluorescence linearity: 2 ±0.05% for chicken erythrocyte nuclei (CEN)
• Fluorescence precision: <3% CV for CEN
• Data acquisition rate: Up to 10,000 events/second
• Signal processing: 24-bit data path

Fluidics

• Flow cell:
• 200-μm ID quartz capillary
• Minimum detectable particle size 0.5 μm
• Minimum acquisition sample volume:
• Standard 12 x 75-mm tubes: 50 μL
• BD Trucount™ tubes: 150 μL
• Loader with standard 12 x 75-mm tubes: 100 μL
• Recommended sheath fluid: 0.2-μm filtered DI water with BD™ Sheath Additive
• Maximum events per sample: 1 million

Installation requirements

• Power requirements: 100–240 VAC, 50/60 Hz
• Typical power consumption: 154 VA
• Heat output: 240 BTU/hour maximum output
• Operating ranges: 15°C–30°C; <80% relative humidity
• Instrument size (H x W x D):
• 11 x 14.75 x 16.5 in.
• (27.9 x 37.5 x 41.9 cm)
• Footprint with fluid bottles (H x W x D):
• 11 x 21.5 x 16.5 in.
• (27.9 x 54.6 x 41.9 cm)
• Weight: 30 lb (13.6 kg)
• Fluid bottle capacity:
• 2 L sheath fluid
• 2 L waste
• 250 mL BD™ Detergent Solution Concentrate
• 250 mL BD™ FACSClean solution
• Noise under normal operating conditions: <60 dBA

BD FACSVia Loader

• Power requirements: No additional power necessary
• Tube compatibility (BD FACSVia clinical software): BD Trucount tubes and 12 x 75-mm tubes accommodated using the supplied 24-tube rack
• With BD FACSVia research software: Standard 96-well (flat, round, and v-bottom) plates in addition to tube types
• Space requirements:
• Minimum bench depth 28 in. (71 cm)
• Minimum width (with Loader) 19.5 in. (49.5 cm)
• Weight: 7 lb (3.2 kg)