BD Biosciences LSRFortessa SORP 3 Lasers: 4Blue/2Violet/3YellowGreen Flow Cytometer
BD Biosciences LSRFortessa SORP 3 Lasers: 4Blue/2Violet/3YellowGreen Flow Cytometer
Recent PM from BD (11/16/22); Certified and Ready for a Service Contract!
Includes:
- HTS (High Throughput Options)
- Windows 7 Pro computer with FACSDiva Software Version 8.0.1
- Installed Lasers:
- Violet Laser 405 nm: 50 mW with 2 PMT detectors (Trigon)
- 525/50 (505LP)
- Z473/10
- Blue 488 nm: 50 mW with 3 PMT detectors plus side scatter (Octagon)
- 710/50 (685LP)
- 582/15 (570LP)
- 488/10
- 675/20 (670LP)
- 515/20 (505LP)
- Yellow Green 561 nm: 50 mW with 3 PMT detectors (Octagon)
- 610/20 (600LP)
- 670/630 (635LP)
- 582/15
Technical Specifications:
- Excitation Optics
- Excitation Optical Platform:The special order BD LSRFortessa optical layout allows for up to five lasers.
- Flow Cell Design:Rectangular Quartz Cuvette: Internal cross-section, 430 x 180 μm
- Fixed optical assembly of the lasers, with up to 5 spatially-separated laser beams.
Emission Optics
- Optical Coupling:The quartz cuvette flow cell is gel coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency.
- Forward Scatter Detection:Photodiode detector with a 488/10 bandpass filter
- Side Scatter Detector:Photomultiplier tube (PMT) with a 488/10 bandpass filter
- Emission Optical Design:The special order BD LSRFortessa cell analyzer uses BD’s patented octagon and trigon detection system. Using reflective optics and fiber-coupled PMTs ensures more efficient light collection than that obtained from transmission optics.
Performance
- Fluorescence Sensitivity:FITC-A: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)
- PE-A: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
- Measurements performed using SPHERO Rainbow Calibration Particles RCP-30-5A
- Fluorescence Resolution:Coefficient of variation PI: area of <3%, full G0/G1 peak for chicken erythrocyte nuclei (CEN) stained with propidium iodide (PI)
- Fluorescence Linearity:Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
- Side Scatter Sensitivity:Achieves separation of 0.5-µm beads from noise in side scatter.
- Forward and Side Scatter Resolution:Scatter performance is optimized for resolution of lymphocytes, monocytes, and granulocytes.
- Forward Scatter PMT Option:A forward scatter PMT upgrade is available for small particle detection through the special order program.
- Data Acquisition Rate:Theoretical maximum electronic data collection of 70,000 events/second.
- Typical operation at 20,000 events/second which requires a sample concentration of 2 x 107 cells/mL at an instrument flow rate of 60 μL/min
Fluidics
- Sample Flow Rates:Front key panel provides three modes: RUN, STNDBY, and PRIME
- Continuously adjustable flow rate, plus three preset flow rates:LO = 12 μL/min
- MED = 35 μL/min
- HI = 60 μL/min
- Standard Fluidic Reservoirs:One 8-L sheath and 10-L waste container provided.
Additional Details
Additional Details
- Manufacturer: BD Biosciences
- Model Name: LSRFortessa SORP 3 Lasers: 4Blue/2Violet/3YellowGreen
- Model Number:
- Product ID: 11370
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