BD Biosciences LSRFortessa SORP Cell Analyzer 5 Lasers: Blue/Red/Violet/YellowGreen/UV Flow Cytometer

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Includes:

• Windows computer with FACSDiva Software
• Installed Lasers:
• Blue 488 nm
• Red Laser 640 nm
• Violet Laser 405 nm
• Yellow-Green 561nm
• UV: 355nm

The BD LSRFortessa cell analyzer puts the power of the BD LSR platform into a compact footprint. It can easily fit on the benchtop for more cost-effective space utilization. 

The instrument delivers the optimal sensitivity and resolution required for multicolor applications. The BD LSRFortessa cell analyzer can be used to detect up to 18 colors simultaneously and supports up to 4 lasers, and is upgradeable to 7 lasers through the special order program. In addition to the reduced size, design innovations make filters and detectors more accessible for easier setup of new experiments.

Through the BD special order program, customers can upgrade the BD LSRFortessa to support more lasers, choosing from 16 different wavelengths and a wide range of powers. This flexibility allows users to configure the instrument to meet their exact requirements for advanced assay development.

Optics Specifications:

• Excitation optics
• Excitation optical platform: The BD LSRFortessa optical layout allows for up to four lasers.
• Laser Options with Powers
• 355 nm: 20 mW
• 405 nm: 50 mW
• 488 nm: 50 mW
• 640 nm: 40 mW
• Optical efficiency: Power loss at flow cell: <20% of specified laser power
• Flow cell designRectangular quartz cuvette: Internal cross-section, 430 x 180 µm
• External quartz cuvette surfaces are anti-reflective coated for optimal transmission of laser light.
• Fixed optical assembly with spatially separated laser beams.
• Emission optics: 
• Optical coupling: The quartz cuvette flow cell is gel- coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency.
• Forward scatter detection: Photodiode detector with a 488/10 bandpass filter
• Side scatter detector: Photomultiplier tube (PMT) with a 488/10 bandpass filter
• Emission optical design: Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays. The BD LSRFortessa uses BD’s patented octagon- and trigon-shaped optical pathways that use signal reflection to maximize signal detection. Please see the separate filter guide for information on dye and filter options.

Performance Specifications:

• Fluorescence sensitivity: FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
• PE-Cy™5: 10 molecules of equivalent soluble fluorochrome (MESF-PE-Cy5)
• APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)
• FITC and PE measurements performed using SPHERO™ Rainbow Calibration Particles
• (RCP-30-5A)
• PE-Cy5 and APC measurements performed using SPHERO Ultra Rainbow Calibration Particles (URCP-38-2K)
• Fluorescence resolution: Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Fluorescence linearity: Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
• Forward and side scatter sensitivity: Enables separation of fixed platelets from noise.
• Forward and side scatter resolution: Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.
• Side scatter resolution: Enables separation of 0.5-µm beads from noise.
• Forward scatter PMT option: A forward scatter PMT upgrade is available for small particle detection through the special order program.
• Data acquisition rate: 40,000 events/s with beads.

Fluidics

• Sample flow rates: Front key panel provides three modes: RUN, STANDBY, and PRIME
• Continuously adjustable flow rate, plus three preset flow rates:
• LO: 12 µL/min
• MED: 35 µL/min
• HI: 60 µL/min
• Standard fluidic reservoirs: One 8-L sheath container and one 10-L waste container provided.
• Recommended fluidics option: BD FACSFlow™ supply system: automated fluidics system, which includes a rolling cart and two 20-L Cubitainer® packages

High throughput option

• The BD High Throughput Sampler (HTS) option is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate.
• The HTS can be front or side mounted on the BD LSRFortessa.
• HTS throughput Acquisition: Less than 15 minutes per microtiter plate in high throughput mode using a 2-second acquisition, less than 44 minutes in standard mode using a 10-second acquisition
• Carryover
• <0.5% HT mode
• <0.75% STD mode

Installation requirements

• Dimensions (H x W x D): 38 x 36 x 30 in. (96.5 x 91.4 x 76.2 cm)
• Weight: ~440 lb (199.6 kg)
• Temperature operating range: 66–79ºF (19–26ºC)
• Humidity: 10% to 90% relative, non-condensing
• Heat dissipation: 2353 BTUs per hour
• PowerOperation at 100/115/230 VAC and 50 or 60 Hz
• Maximum power: 1,500 watts
• Noise: <70 dB
• Air supply: None required
• Electrical requirements: BD requires one dedicated circuit for the cytometer and the computer system (including printer) with a dedicated AC source not shared with any other equipment. The instrument will be powered from the line conditioner supplied by BD Biosciences