Visitron SRRF: Super Resolution Algorithm Module Software:Microscope

Visitron VisiSRRF: Super Resolution Algorithm Module

The Visitron VisiSRRF (Super-Resolution Radial Fluctuations) Algorithm Module is an advanced, software-based super-resolution imaging solution that enhances fluorescence microscopy beyond the diffraction limit. Unlike traditional super-resolution techniques, VisiSRRF works with any fluorescence microscope and does not require specialized fluorophores or additional hardware. This module is optimized for live-cell imaging, offering real-time super-resolution without excessive phototoxicity or long exposure times.

Key Features:

• Super-Resolution Imaging – Achieves resolution down to ~70 nm on standard fluorescence microscopes.
• Live-Cell Compatibility – Low-intensity imaging minimizes phototoxicity and photobleaching.
• Real-Time SRRF Acquisition – Integrated with VisiView for on-the-fly processing and post-processing analysis.
• Compatible with Any Fluorophore – Works with both standard and blinking fluorophores for enhanced resolution.
• Optimized for Widefield and Spinning Disk Microscopy – Improves imaging across multiple modalities.
• Faster and More Convenient – Based on the nanoJ-SRRF algorithm but optimized for speed and usability.

Why Choose VisiSRRF for Live-Cell Imaging?

• No Need for Sparse Molecule Distribution – Works with dense fluorophore staining.
• Compatible with Conventional Fluorophores – No special probes required.
• Ultra-Low Excitation Power – Operates at 2 × 10⁻⁴ kW/cm² to 2 × 10⁻¹ kW/cm², reducing cell stress.
• Fast Image Acquisition – Captures ~100 frames at low exposure (~10ms) to retain live-cell dynamics.

Applications:

• Super-Resolution Microscopy (SRRF-based imaging)
• Live-Cell Imaging – Captures fine structural details while preserving cell viability.
• Single-Molecule Localization – Enhances subcellular structure visualization.
• Fluorescence Microscopy Enhancement – Extends resolution limits of conventional microscopes.
• Comparison with 3D Deconvolution – Provides better resolution without requiring point spread function (PSF) estimation.

Specifications: